Gel-permeation chromatography on Sephadex LH-20, using ethanol as eluent, permits the resolution of neutral hydroxy lipids according to molecular size.The influence ORG RAW HAZELNUTS of molecular shape, functional groups, chain lengths, and degree of unsaturation, as well as the effect of the eluent on the elution pattern are discussed.The usefulness of the method for the separation of classes of hydroxy lipids NIPPLE CREAM which cannot be resolved by other chromatographic procedures, is demonstrated.
Examples include the separations of 1,2- and 1,3-diglycerides from long-chain alcohols, and of alkyl ethanediol monoethers from cholesterol.